Saudi Journal of Medical and Pharmaceutical Sciences (SJMPS)
Volume-5 | Issue-10 | 907-910
Original Research Article
Prunus Domestica L.: A Domestic Source of Natural Antioxidants
Hina Imran, Mehreen Latif, Zahra Yaqeen, Tehmina Sohail, Syed Rafay Yaqeen, Syed Shafay Yaqeen, Wasif Iqbal
Published : Oct. 30, 2019
Abstract
The aim of this study is to evaluate crude extract, ethyl acetate, chloroform and butanol fractions of P. domestica for their in vitro antioxidant activities using 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging and reducing power assay on 1.25%, 2.5% and 5% concentrations. According to the results fraction of ethyl acetate showed maximum free radical scavenging up to 94% at the concentration of 5%, 93% at the concentration of 2.5% and 67% at the concentration of 1.25% followed by crude extract that showed 85, 54 and 41% activity at the concentrations of 5, 2.5 and 1.25% respectively. Chloroform fraction showed 70, 55 and 39% scavenging activity at 5, 2.5 and 1.25% concentrations respectively. While butanol fraction exhibited least activity i.e. 39, 36 and 9% on 5, 2.5 and 1.25% concentrations respectively. On the other hand, by reducing power assay method, ethyl acetate exhibited 90, 70 and 55% percent reducing power, followed by crude extract which exhibited 84, 62 and 41%, while chloroform extract exhibited 70, 42 and 28% and the least activity was shown by butanol extract i.e. 45, 22 and 12% at the concentrations of 5, 2.5 and 1.25% respectively. This study showed that ethyl acetate fraction exhibited best antioxidant potential and can be further isolated for biologically active constituents for further studies.