Objective: To assess nurses’ knowledge and evaluate their practice for prevention of infection among burn patients, also to suggest guidelines especially of nursing practice for prevention of infection in burn patients. Study Design: A descriptive cross sectional study. Place and Duration: Six months, from April 2017 to Sep 2017, in burn units of 03 selected hospitals of Lahore. Methods: The study included all nurses who were providing care to burn patients (N=48). Nurses’ knowledge was assessed by using a self-structured questionnaire and their practices were evaluated by direct monitoring using an observational Checklist. Results: Females constituted 100% of study participants. 62.5% of nurses had a GNM whilst 37.5 % had a Nursing degree. 73% (35) of participants had less than 2 years of experience working in the Burns Unit. Knowledge regarding infection control was received by 89% of nurses but their source of information was practice not in-service educational programs. Consequently they had unsatisfactory level of knowledge and practices indicated by knowledge and practice score less than 75%. Conclusion: The findings of the current study revealed nurses’ low level of knowledge and practices. Hence, healthcare settings are required to organize advanced training sessions and to develop unit specific clinical guidelines and protocols.