Several chemical compounds are used worldwide to induce diabetes in animals. Alloxan monohydrate and streptozotocin are among those are commonly used because of their cheap coast. However, other chemical compounds are less toxic but more expensive. This pilot study was conducted to use alloxan as diabetes inducer, and to determine the suitable dose which can make a diabetic albino mice model. The type of induced diabetes was also differentiated by using glimepiride tablets. Three mice were injected with three doses of alloxan monohydrate intraperitoneally (150mg-100mg-150mg)/kg in two days intervals respectively in order to induce and maintain diabetes. The fasting blood sugar measured before and after each alloxan injection. Results showed an increasing in blood glucose levels from an average of 81 mg/dl to 168.6 mg/dl after the first dose (150 mg /kg) of alloxan, after 2nd dose (100 mg /kg) of alloxan from 117.83 mg/dl to 154.67 mg/dl, and from 154.67 mg/dl to 173 mg/dl after 3rd dose (150 mg/kg). On the other hand the glimepiride which was given to mice orally caused dramatic decrease in blood sugar level from 173 mg/dl to 93.67 mg/dl after 4 hrs of administration. From this study it was conducted that the use of different doses of alloxan can induce and maintain diabetes and the induced diabetes is type II. Further work is in progress to examine the use of plant extracts in order to regulate blood sugar levels in induced diabetic mice.