The present study assessed the alteration of the liver biochemical enzymes following dermal exposure to petroleum motor spirit (pms) on 25 albino whister rats. 10 served as control while the treated had 15 albino whister rats and clustered into 3 based on the treatment scheme (1ml, 2ml, and 3ml). The serum biochemical assays, body weight, and histopathology of the liver where evaluated. The study revealed the mean of 101.20±2.781 and 111.40±2.591 for weight before and after treatement respectively with a statistically significant (t= -20.821, df= 9, p=<0.05) for control subjects. While the mean of the treated group (PMS) was 116.67±12.199 and 110.33±11.872 for weight before and after treatment correspondingly. The study demonstrated a statistically significant mean difference between the two different weights for pre and post treatment exposure. In analyzing the biochemical assays, the result of treatment with 1ml PMS showed a moderate increase in ALP (11.60±.548) when compared to the control (11.40±0.516), while AST and ALT significantly decreased (192.00±19.170 and 110.80±9.311) when compared to the control (245.40±4.648 and 143.60±13.426) at P<0.05. When treated with 2ml PMS the ALP, AST, ALT (12.60±.548, 298.20±29.029 and 172.80±20.266) all increased when compared to the control (11.40±0.516, 245.40±4.648 and 143.60±13.426) at p<0.05. The mean comparison of the treatment of albino whister rats using 3ml PMS showed a significant increase in ALP and AST (14.00±2.739 and 275.40±30.672) when compared to the control (11.40±0.516 and 245.40±4.648) at p<0.05. But, comparing the obtained result of ALT, result showed a decrease in the level of ALT (118.00±27.386) when compared to control (143.60±13.426). The histopathological examination results as described in the histopathology report shows significant suggestion of deformation in the cell structure of hepatocytes subsequent to treatment with varying doses of PMS. Dermal exposure of these xenobiotics is almost certainl