Background: Lip prints can be used in crime investigation to establish the presence of a victim or a suspect at a crime scene since they are visible with the naked eye but latent prints are not visible with naked eyes. The examination of these features is called cheiloscopy. Cheiloscopy or Quiloscopy is simply the study of the patterns produced by the lip prints and their application. Aim: This study was aimed at investigating the association between lip prints types and gender and the proportionality difference in distribution of lip prints between male and female. This study will add to the body of knowledge information on the association of gender and lip print which will be beneficial to geneticists, forensic experts, biologists and everyone who has interest in security outfits. Materials and Methods: A cross-sectional study design with 450 volunteers. Volunteer subjects were conveniently selected from across Port Harcourt Metropolis. Results and Discussions: This study suggest that lip distribution is independent of the gender and what was seen in the upper right quadrant (URQ) could be by a coincidence and not strong enough to suggest that lip print distribution is dependent on gender. Conclusion: The findings of this study suggest that Type I is the most predominant lip print type, followed by Type II among the study population. The study showed that not all quadrants exhibited gender differences in the pattern of lip prints distribution. Only a one quadrant (Upper Right Quadrant) of lip prints can be said to have gender discriminatory indicators, and this maybe a mere coincidence. Thus, suggests that lip prints may not be a good predictive tool for gender.