Background: Regenerative endodontic treatment uses a scaffold within a disinfected canal to support healing in immature permanent teeth. This case series describes the use of two scaffold approaches in mandibular premolars. Case Presentations: A 12-year-old girl received treatment of tooth 34 using an induced intracanal blood clot. A 12-year-old boy received treatment of tooth 45 using platelet-rich fibrin (PRF). At the first visit, both canals were irrigated with sodium hypochlorite and medicated with antibiotic paste. At the second visit, the medicaments were removed and the canals were irrigated with EDTA. Bleeding was induced beyond the apex of tooth 34 and allowed to clot; PRF was placed in tooth 45. Mineral trioxide aggregate was placed over each scaffold, followed by coronal restoration. Immediate postoperative, two-week, and three-month follow-up radiographs documented the treated teeth. Conclusion: These cases illustrate the use of an induced blood clot and PRF as scaffolds in regenerative endodontic treatment. Radiographic follow-up was available up to three months. Longer clinical and radiographic follow-up is needed to assess sustained healing and continued root development, and these two cases cannot establish comparative scaffold effectiveness.