Glycated Hemoglobin (Hb A1c) is now used largely for DM control. Hb A1c is influenced by many risk factors; these include BMI, physical activity, age, ethnicity, diet and smoking. The determinants of Hb A1cvalues in Sudanese have not been studied despite their utmost value in DM control. The objective of this study is to evaluate age, gender and body mass index as determinants of glycated hemoglobin in non-diabetic Sudanese population. A cross sectional study was conducted during 2016-2018 in Sudan covering Khartoum state, Northern state, Gezira state, Red Sea state and North Darfur state, on adults of ages between 20-60 years and not known to be diabetic or suffering from any chronic illness.1097 participants were assessed by a questionnaire covering age, gender and other demographic and social characteristics. BMI was calculated after measuring weight and height by standard scales. A sample of 5 ml venous blood was taken for FBG to exclude DM and measurement of Hb A1c using a modified ELISA reader known as Cobas Integra 800 (Roch) machine. Correlations between the variables were estimated and P value < 0.05 was considered statistically significant. There is a positive correlation between HbA1c and gender; female subjects had higher values than male subjects. The mean of HbA1c in females was 4.6 ±0.9 % which was more than the mean in males 4.5 ±1.0 % with significant p value of 0.03. There are slight differences in HbA1c levels between the age groups, the oldest (more than 50 years) having the lowest values (4.4%) and those with group of age between 35-50 years have the highest value (4.7%). HbA1c levels were found to be higher (4.8%) in those having BMI more than 30. There was no significant correlation between HbA1 c; age, weight and BMI (r =.009, .010, =-.032) and the p values were 0.7, 0.7 , 0.33.respectively . There was intermediate correlation between HbA1c and Height (r) =0.13 with significant p value of 0.00. This study showed significant higher HbA1c levels in females compared to males. Age and BMI have no significant effect on the HbA1c levels in Sudanese population but there was a significant correlation with height.