Lutein as a carotenoid, provides a wide range of nutritional and health benefits due to their multifarious biological impacts on humans, which include antioxidative, immunomodulatory, and anti-inflammatory properties. This study was carried out to evaluate the Cognito motor modulatory function of lutein on Hippocampal cellular Architecture in Diazepam induced memory impairment in Wistar rats. Thirty (30) male rats were used for this study and the rats were acclimatized for a period of 14 days, and was then divided into six groups; 1 (Control), 2 (Diazepam 5mg/kg), 3 (Diazepam + Lutein 20mg/kg), 4 (Diazepam + Lutein 40mg/kg), 5 (Diazepam + Lutein 60mg/kg), and 6 (Diazepam + Donepezil - Standard drug) for a period of 21 days. The catalase level was seen to be statistically significant (p<0.05) when the group 2 (diazepam only treated) and group 3 were separately compared to that of the control group (group 1). In neurobehavioural test conducted, Group 2 in handgrip stability time test conducted showed a significant (p<0.05) decrease signifying a lack of peak force for handgripping when compared with group 1(control group), this was made significant by the increase in the number of incorrect hole entry by group 2 wistar rats in Barnes maze test in week 1 and 2 of the study period, Lutein showed a motor modulatory function in the brain through its antioxidizing and anti-proteolytic properties, with its dose dependence effect on group 3 Wistar rats, of which catalase concentration level seems to be lesser when compared to group 2.