This cross-sectional comparative study was done at radiotherapy department of Rajshahi Medical College Hospital, Rajshahi from July, 2009-June, 2010. A total number of 60 diagnosed cases of carcinoma larynx (Histologically squamous cell carcinoma) were enlisted and were divided into study group e.g. Chemo-Radiotherapy group (Group A) consisting 30 patients were treated by sequential chemo-radiotherapy with two cycles of cisplatin (20mg/m2) & 5-FU (500mg) per day for 4 days 3 weeks apart followed by radiotherapy after 1½ weeks at 60-66 Gray in 30-33 fractions in 6-6½ weeks and control group e.g. radiotherapy group (Group B) consisting 30 patients were treated with conventional radiotherapy of 60-66 Gray in 30-33 fractions in 6-6½ weeks. The objective of the study was to observe and compare the results of treatment & side effects of treatment on both arms during & after treatment. In chemo-radiotherapy group (Group-A) complete response (CR) was found in 20 cases (66.67%) and partial response (PR) was found in 10 cases (33.33%). In radiotherapy group (Group-B) complete response (CR) was found in 12 cases (40%) and partial response was found in 18 cases (60%). It was found that complete & partial response was 100%. There was no death or no major complications throughout the study period. Calculated 2 value was 4.28 was greater than the table value 2 i.e. 3.84. It corresponds to probability of 0.05 in 2 table (P < 0.05). Hence treatment of laryngeal cancer with sequential chemo-radiotherapy was statistically significant.