Background: Lipids are essential in many facets of life, and the ABO system is the paramount system in the transfusion process. This research seeks to correlate serum levels of total cholesterol, triglycerides, HDL-C, LDL-C, and ABO blood type in healthy Sudanese adults. Methods: A cross-sectional community-based study was undertaken in Khartoum State, Sudan, from March 2020 to August 2021. Two hundred healthy people were enlisted, including 50 from blood group A, 50 from blood group O, 50 from blood group B, and 50 from blood type AB. The blood lipid profile was assessed using an auto chemistry analyzer, and statistical analysis was conducted using SPSS version 25. Results: A significantly elevated serum cholesterol level (p value = 0.004) and triglycerides (p value = 0.000) were seen across various blood types, with the AB blood group exhibiting the highest levels of both serum cholesterol and triglycerides. There exists a positive link between serum cholesterol levels and age in blood groups O, B, and AB, with correlation coefficients of r=0.399 (P=0.004), r=0.431 (P=0.002), and r=0.403 (P=0.004), respectively. A positive association exists between serum triglycerides and age in blood groups A and O, with coefficients r=0.463 (P=0.001) and r=0.408 (P=0.003), respectively. There exists a significant association between LDL-C and age in blood groups B and AB (r=0.385, P=0.006; r=0.463, P=0.001, respectively). Conclusion: Individuals with the AB blood group have elevated serum cholesterol and triglyceride levels compared to other blood groups, with a positive association shown between age and cholesterol, triglycerides, and LDL-C across various blood types.