Umbilical cord blood banking is a rapidly growing field with significant medical potential, especially in regenerative medicine and hematopoietic stem cell transplantation. The first successful Umbilical cord blood transplant in 1988 marked a breakthrough, leading to the establishment of both public and private Umbilical cord blood banks worldwide. Pharmacy students, as future healthcare providers, play a pivotal role in patient education regarding Umbilical cord blood banking, but research on their knowledge levels remains sparse. This study was conducted at JKK Nattaraja College of Pharmacy over six months using a pre-post intervention design to assess the impact of education on students' knowledge and aptitude regarding umbilical cord blood banking. Initially, a structured questionnaire was administered to assess baseline knowledge. After an educational intervention comprising lectures and discussions, a post-survey assessed changes. Results indicated significant improvement in awareness and understanding of Umbilical cord blood banking applications, benefits, and collection methods. Before the intervention, most students lacked clarity on stem cell sources, Umbilical cord blood uses, and public vs. private banking differences. After the session, understanding improved markedly, with over 90% correctly identifying medical applications and banking types. The study concludes that educational programs are essential for enhancing pharmacy students’ awareness and aptitude toward UCB banking, preparing them to counsel patients effectively.