Antimicrobial resistance (AMR) represents a profound and escalating global health crisis, threatening to dismantle the foundations of modern medicine. In response, healthcare systems worldwide have implemented antimicrobial stewardship programs (ASPs) to optimize antimicrobial use, improve patient outcomes, and curb the emergence of resistant pathogens. This review article explores the central thesis that the efficacy and success of contemporary ASPs in tertiary care settings are not merely enhanced by but are fundamentally dependent on the synergistic and deeply integrated collaboration of pharmacists, laboratory specialists, and nurses. We dissect the unique and indispensable contributions of each profession: the pharmacist's expertise in medication optimization, prospective audits, and formulary management; the laboratory specialist's foundational role in rapid diagnostics, susceptibility testing, and data-driven guidance; and the nurse's critical frontline position in timely administration, clinical monitoring, and patient education. By examining the intricate workflows and communication pathways that bind these roles, we present a model for an ideal "stewardship handshake," illustrating how their combined efforts lead to targeted, effective, and safe antimicrobial therapy. Furthermore, this review addresses the significant barriers to effective interprofessional collaboration—such as communication silos, hierarchical structures, and workflow inefficiencies—and proposes evidence-based solutions to overcome them. We also delineate key performance indicators for measuring the collective impact of this collaborative model. Finally, we look toward the future, considering the integration of emerging technologies like artificial intelligence and telehealth into stewardship, and conclude with a call to action for healthcare institutions to recognize, foster, and invest in these interprofessional teams as the primary defense in preserving the viability of antimicrobials for generations to come.