Background: The medical devices sector in India plays a crucial role in the country's healthcare system, ranging from simple bandages to complex devices like CT scans. Materiovigilance is a system for monitoring adverse events related to medical devices. The program empowers healthcare professionals to report adverse events, ensuring patient safety and improving the overall quality of healthcare through systematic surveillance and data analysis. Objective: To assess knowledge, attitude and practice of healthcare professionals toward materiovigilance and evaluate the impact of an educational intervention on these aspects. Methods: A cross-sectional prospective questionnaire study was conducted for a period of six months at a tertiary care teaching hospital with a sample size of 307 healthcare professionals. Healthcare professionals from Medical, Nursing, Pharmacy, Dental and Physiotherapy were included in the study. Base line data was collected by administering questionnaire. Educational intervention was provided to the same study participants and post interventional data was also collected. Chi-square test was used to test the significance of impact of educational intervention. Results: The collected data showed that 94.46% (290) were under the age group of 18-28. Before the intervention, only 36.16% (141) were aware of the term materiovigilance which increased to 100% (307) after the intervention. Before the intervention, 65.47% (201) did not know where the NCC of MvPi is located and 16.94% (52) gave the wrong answer, whereas after the intervention 88.6% (272) participants gave the right answer. Conclusion: Our study identified a significant knowledge gap in materiovigilance among healthcare professionals before the educational intervention. After the intervention, participants demonstrated a substantial increase in their knowledge and awareness of medical device-related adverse events (MDAEs).