The indiscriminate use of pesticides in agriculture has led to the contamination of aquatic ecosystems, adversely affecting non-target organisms such as freshwater fish. This study investigates the behavioral responses of the freshwater fish Channa punctatus to sub-lethal concentrations of the synthetic pyrethroid pesticide, fenvalerate. The results reveal significant alterations in swimming behavior, respiratory patterns, feeding activity, and overall responsiveness, indicating neurotoxic and physiological stress. These behavioral changes may impair the survival and ecological fitness of the species, emphasizing the need for stringent regulation of pesticide usage.