Sunscreen is a material that can absorb or reflect ultraviolet (UV) radiation to protect the skin from the negative impact. Sunscreen derived from synthetic compounds can cause long-term toxic side effects, so natural materials can be a safer alternative to sunscreen. Basil leaves (Ocimum basilicum L.), and jicama tubers (Pachyrhizus erosus L.) have sunscreen activity because they contain flavonoids that absorb UV. This study aims to compare the sunscreen activity of basil leaf extract cream with jicama tuber starch cream and determine the concentration of basil leaf extract or jicama tuber starch with the highest SPF value. Basil leaves were extracted by maceration using ethanol solvents, while jicama tubers were made into starch powder. Basil extract and jicama starch were formulated into a cream preparation. Then, an evaluation of the preparation was carried out. The SPF value was determined in vitro using a UV-Vis spectrophotometer instrument at a wavelength of 290 to 320 nm. The results of the evaluation of the cream preparation meet the requirements for the entire test. The results of measuring the SPF value of basil leaf extract sunscreen cream with concentrations of 15%, 20%, and 25% were 9.40 (maximum), 10.66 (maximum), and 15.41 (ultra). The results of measuring the SPF value of jicama tuber starch sunscreen cream with concentrations of 15%, 20%, and 25% were <2, so it is ineffective as a sunscreen. Basil leaf extract sunscreen cream with a concentration of 25% showed the best SPF value with an ultra sunscreen ability level.