Fruits are medicinal and also contain essential phytonutrients that gives the fruits potency that keeps the body healthy. The aim of the study is to ascertain the Effects of Smoothies (banana, apple and pineapple) on Oxidative Stress Markers following administration of monosodium glutamate in Male Wistar Rats. Thirty (30) animals weighing 130kg to 180kg were randomly selected into 6 groups with 5 animals per group. Group 1 received 5mls of distilled water, group 2 received 1ml/kg (low dose) of smoothies, group 3 received 2ml/kg (medium dose), group 4 received 3ml/kg (high dose), group 5 received 400mg/kg of monosodium glutamate, group 6 received 400mg/kg of monosodium glutamate and 3ml/kg (high dose) of smoothies co-administered. Administration was carried out for 14 days and on the 15th day, the animals were sacrificed, semen was harvested and 5ml blood was collected via cardiac puncture. Statistical analysis was done using ANOVA and expressed as Mean±SEM. Statistically P < 0.05 was said to be significant. SPSS version 26 was used. The results showed significant increase in SOD in the group that was administered smoothies (high dose) and decrease in the group that was administered 400mg of monosodium glutamate (MSG). MDA increases in the groups that was administered MSG alone and when co-administered with smoothies (high dose). MDA also decreases in the groups given medium and high dose smoothies. Catalase decrease in the group that was given MSG alone. Gluthione increases in the groups that was given medium and high dose smoothies and decrease in the group that was given MSG only.