Morinda citrifolia is an herbal medicine that is used for disease treatment as recommended in traditional medicine. The present study aimed to characterize silver nanoparticles from Noni seed. In this experimental study, green synthesis was carried out then the synthesized nanoparticles were characterized using SEM, EDX, TEM and FTIR. Findings showed that SEM images of the AgNPs of different shapes were obtained in the case of different seed extracts being used as reducing and capping agents. Seed extract formed approximately spherical, triangular, and cuboidal AgNPs, respectively. Elemental mapping of AgNPs by SEM-EDX shows the presence of 0.31% Ag and 40% oxides with 39% Carbon and other elements in trace amounts. TEM image demonstrates that the AgNPs were spherical. The image shows agglomerates of small grains and some dispersed nanoparticles, confirming the results obtained by SEM. FTIR result reveals the assignment of functional groups to 19 FTIR bands (690-3833 cm 1). Bands were categorized based on possible functional group classes (alkene, aromatic, alcohol/phenol/ether, amine, etc.). Specific functional groups like alkenes, conjugated alkenes, ketones, aldehydes, nitriles, alkynes, and carboxylic acids were identified based on characteristic wavenumbers. The study presents a green synthesis approach to prepare silver nanoparticles using Noni seed extract. Reduction of silver nitrate with Noni seed extract is a simple, conducted at room temperature, efficient, and clean method to synthesize silver nanostructures.