This work was initiated with an objective that would screen for bioactive specifically antibacterial molecules secreted by actinomycetes growing in fresh water pond soil. Actinomycetes being treasure trove of thousands of bioactive molecules. It is recommended that either novel or present day bio molecule derivatives should be detected and pertinent drug development process be carried out with this background this study was proceeded to identify strains of Microbacterium barkeri (LMA4), Corynebacterium argetoratense (LMA5) and Streptomyces shenzhenensis (LMA6) form the local marshy pond soil. The actinomycetes was with Arial mycelia was gram positive with profuse branching. In a test considering MDR strain of Escherichia coli (BME4) and Staphylococcus aureus (BMS4) as test bacteria. The 16s rRNA sequences and the BLASTn analysis helped out to predict the sequence identification as the actinomycetes isolates.