Background: Male sexual dysfunction is a group of disorders that affect sexual function, most notably erectile dysfunction (ED), Peyronie's disease (PD), and premature ejaculation (PE). More than 50% of men between the ages of 40 and 70 report having some form of erectile dysfunction, which is a rather high prevalence that rises with age. Age, diabetes mellitus (DM), cancer, stroke, hypertension, penile trauma, depression, anxiety, and disturbances in central serotonin neurotransmission and 5-HT postsynaptic receptor function are risk factors for male sexual dysfunction. The International Index of Erectile Dysfunction, the Sexual Health Inventory for Men, and the Premature Ejaculation Diagnostic Tool are three sexual questionnaires that can be used to screen for these illnesses. Fenugreek, or Trigonella foenum-graecum L., also known as methi (in Hindi), has been used as a culinary spice, flavouring ingredient, and medicinal plant for a long time. Despite being more well-known for its seeds, fenugreek leaves and stems have also been claimed to offer medical benefits, including laxative, lactation stimulant, and labour assist properties. Method: The purpose of the current study was to assess the efficiency of flavonoids and steroid saponin found in Trigonella foenum-graecum seed for their inhibitory influence on PDE-5 enzyme to elicit the aphrodisiac potency. The Auto Dock software used a grid-based docking algorithm to determine the bond. Result: Fenugreek found to be effective aphrodisiac agent and effectively binds to be target protein PDE-5 with binding energy -9.8, -10.6, -7.88 & -6.61 kcalmol-1for Diosgenin, gitogenin, Naringenin and Vitexin respectively. Conclusion: The outcome of findings revealed that steroidal saponin(diosgenin) and flavonoid(vitexin) showed potent inhibitory effect on PDE-5 enzyme which reflects the efficacy of fenugreek seed as potent aphrodisiac agent via synergetic effect of steroidal saponin and flavonoid.